Pyridine Extraction of Nocardial Acid Fastness
نویسندگان
چکیده
منابع مشابه
Elimination of the acid fastness but not the gram positivity of leprosy bacilli after extraction with pyridine.
Bacilli from a number of tissues excised from cases of lepromatous and borderline leprosy, unlike mycobacteria, were found to lose their acid-fastness after treatment with pyridine.
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Acid-fastness is usually defined by the Ziehl-Neelsen technique: staining in carbol-fuchsin, differentiation in HCl-alcohol, and counterstaining in methylene blue.8 As a result, acid-fast substances are red, non-acid-fast materials, blue. Some authors have felt that this property of differential staining resided in one specific type of compound, the mycolic acid type of long-chain fatty acid fo...
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BACKGROUND Histoplasmosis (HP) is diagnosed by visualizing intracellular microorganisms in biopsy and/or culture. Periodic-acid Schiff (PAS) and Gomori methenamine silver (GMS) staining methods are routinely used for identification. The acid-fast property of Histoplasma was identified decades ago, but acid-fast staining has not been practiced in current surgical pathology. Awareness of the acid...
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IN THE TITLE COMPOUND (SYSTENATIC NAME: 3,4,5-trihy-droxy-benzoic acid pyridine monosolvate), C(5)H(5)N·C(7)H(6)O(5), the gallic acid mol-ecule is essentially planar (r.m.s deviation = 0.0766 Å for non-H atoms) and is linked to the pyridine mol-ecule by an O-H⋯N hydrogen bond. An intra-molecular O-H⋯O hydrogen bond occurs in the gallic acid mol-ecule. The gallic acid and pyridine mean planes ma...
متن کاملCytochemical reactions of human leprosy bacilli and mycobacteria: ultrastructural implications.
Leprosy bacilli harvested from freshly biopsied tissue from cases of lepromatous, borderline and histoid leprosy were, in conjunction with Mycobacterium lepraemurium and representative mycobacteria, examined cytochemically with and without their pyridine-extractable acid-fastness. Unlike the mycobacteria, unextracted leprosy bacilli failed to give a positive response to the periodic acid Schiff...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1973
ISSN: 0003-6919
DOI: 10.1128/aem.26.3.426-428.1973